FPGEE for National Association of Boards of Pharmacy (NABP) Practice Exam 2025 - Free NABP Practice Questions and Study Guide

The high-energy molecule involved in acetylation is Acetyl CoA. This molecule plays a critical role in various metabolic pathways, primarily in the transfer of acetyl groups to different substrates, facilitating the process of acetylation.

Acetyl CoA is generated from the breakdown of carbohydrates, fats, and proteins, and serves as a key metabolic intermediate that donates its acetyl group during various biochemical reactions. Acetylation often involves the modification of proteins and other molecules by the addition of an acetyl group, influencing their activity and function.

Other choices like ATP are also involved in cellular energy transfer, but they are not directly related to the acetylation process. NADH is primarily involved in redox reactions and energy transfer from metabolic pathways to the electron transport chain. Coenzyme A, while necessary for forming Acetyl CoA and participating in acetylation indirectly, does not itself act as the high-energy acetyl donor during this process. Thus, Acetyl CoA is the correct answer for a high-energy molecule specifically associated with acetylation.